Genetic examination and mass balance analysis of pyruvate/amino acid oxidation pathways in the hyperthermophilic archaeon Thermococcus kodakarensis. Kenta Nohara, Izumi Orita, Satoshi Nakamura, Tadayuki Imanaka, Toshiaki Fukui. J. Bacteriol., 196, 3831-3839 (2014)
Characterization and gene deletion analysis of four homologues of group 3 pyridine nucleotide disulfide oxidoreductases from Thermococcus kodakarensis. Phurt Harnvoravongchai, Hiroki Kobori, Izumi Orita, Satoshi Nakamura, Tadayuki Imanaka, Toshiaki Fukui. Extremophiles, 18, 603-616 (2014)
Characterization of two members among the five ADP-forming acyl coenzyme A (acyl-CoA) synthetases reveals the presence of a 2-(imidazol-4-yl)acetyl-CoA synthetase in Thermococcus kodakarensis. Tomotsugu Awano, Anja Wilming, Hiroya Tomita, Yuusuke Yokooji, Toshiaki Fukui, Tadayuki Imanaka, Haruyuki Atomi. J. Bacteriol., 196, 140-147 (2014)
Thermostable alcohol dehydrogenase from Thermococcus kodakarensis KOD1 for enantioselective bioconversion of aromatic secondary alcohols. Xi Wu, Chong Zhang, Izumi Orita, Tadayuki Imanaka, Toshiaki Fukui, Xin-Hui Xing. Appl. Environ. Microbiol., 79, 2209-2217 (2013)
Overview of the genetic tools in the Archaea Haruyuki Atomi, Tadayuki Imanaka, Toshiaki Fukui. Front Microbiol., 3, 337 (2012)
Application of a novel thermostable NAD(P)H oxidase from hyperthermophilic archaeon for the regeneration of both NAD+ and NADP+. Xi Wu, Hiroki Kobori, Izumi Orita, Chong Zhang, Tadayuki Imanaka, Xin-Hui Xing, Toshiaki Fukui. Biotechnol. Bioeng., 109, 53-62 (2012)
Characterization of NADH Oxidase/NADPH Polysulfide Oxidoreductase and Its Unexpected Participation in Oxygen Sensitivity in an Anaerobic Hyperthermophilic Archaeon. Hiroki Kobori, Masayuki Ogino, Izumi Orita, Satoshi Nakamura, Tadayuki Imanaka, Toshiaki Fukui. J. Bacteriol., 192, 5192-5202 (2010)
Methionine sulfoxide reductase from the hyperthermophilic archaeon Thermococcus kodakaraensis, an enzyme designed to function at sub-optimal growth temperatures. Eiji Fukushima, Yasuhiro Shinka, Toshiaki Fukui, Haruyuki Atomi, Tadayuki Imanaka. J. Bacteriol., 189, 7134-7144 (2007)
A novel ADP-forming succinyl-CoA synthetase in Thermococcus kodakaraensis structurally related to the archaeal NDP-forming acetyl-CoA synthetases. Kenichi Shikata, Toshiaki Fukui, Haruyuki Atomi, Tadayuki Imanaka. J. Biol. Chem., 282, 26963-26970 (2007)
Disruption of a sugar transporter gene cluster in a hyperthermophilic archaeon using a host/marker system based on antibiotic resistance. Rie Matsumi, Kenji Manabe, Toshiaki Fukui, Haruyuki Atomi, Tadayuki Imanaka. J. Bacteriol., 189, 2683-2691 (2007)
Phosphoenolpyruvate synthase plays an essential role for glycolysis in the modified Embden-Meyerhof pathway in Thermococcus kodakarensis. Hiroyuki Imanaka, Atsushi Yamatsu, Toshiaki Fukui, Haruyuki Atomi, Tadayuki Imanaka. Mol. Microbiol., 61, 898-909 (2006)
Characterization of a novel glucosamine-6-phosphate deaminase from a hyperthermophilic archaeon. Takeshi Tanaka, Fumikazu Takahashi, Toshiaki Fukui, Shinsuke Fujiwara, Haruyuki Atomi, Tadayuki Imanaka. J. Bacteriol., 187, 7038-7044 (2005)
生分解性ポリマーの微生物合成に関する研究
Microaerobic insights into production of polyhydroxyalkanoates containing 3-hydroxyhexanoate via native reverse β-oxidation from glucose in Ralstonia eutropha H16. Kai-Hee Huong, Izumi Orita, Toshiaki Fukui. Microbial Cell Factories.,(2024) https://doi.org/10.1186/s12934-024-02294-4
Gas fermentation combined with water electrolysis for production of polyhydroxyalkanoate copolymer from carbon dioxide by engineered Ralstonia eutropha. Gabriele Di Stadio, Izumi Orita, Ryuhei Nakamura, Toshiaki Fukui. Bioresource Technology., (2024) https://doi.org/10.1016/j.biortech.2023.130266
(R/S)-lactate/2-hydroxybutyrate dehydrogenases in and biosynthesis of block copolyesters by Ralstonia eutropha. Shizuru Ishihara, Izumi Orita, Ken’ichiro Matsumoto, Toshiaki Fukui. Appl. Environ. Microbiol., (2023) https://doi.org/10.1007/s00253-023-12797-6
C1微生物の代謝改変によるメタノールを原料とした生分解性共重合ポリエステルの生合成 Izumi Orita,Toshiaki Fukui. Journal of the Japan Petroleum Institute., 65(6), 213-220(2022) https://doi.org/10.1627/jpi.65.213
Biosynthesis of Poly(3-hydroxybutyrate- co-3-hydroxyhexanoate) From Glucose by Escherichia coli Through Butyryl-CoA Formation Driven by Ccr-Emd Combination. Shu Saito, Ryu Imai, Yuki Miyahara, Mari Nakagawa, Izumi Orita, Takeharu Tsuge, Toshiaki Fukui. Front Bioeng Biotechnol., 10:888973(2022)
Biosynthesis of Polyhydroxyalkanoate Terpolymer from Methanol via the Reverse β-Oxidation Pathway in the Presence of Lanthanide. Izumi Orita, Gento Unno, Risa Kato, Toshiaki Fukui. Microorganisms., 10(1), 184(2022)
Biosynthesis of Poly(3-hydroxybutyrate- co-3-hydroxyhexanoate) from CO2 by a Recombinant Cupriavidusnecator. Kenji Tanaka, Kazumasa Yoshida, Izumi Orita, Toshiaki Fukui. Bioengineering (Basel)., 8(11), 179(2021)
Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis. Ryoga Fujiwara, Rikako Sanuki, Hiroharu Ajiro, Toshiaki Fukui, Shosuke Yoshida. Sci Rep., 11(1), 19991(2021)
Isopropanol production with reutilization of glucose-derived CO 2 by engineered Ralstonia eutropha. Dyah Candra Hapsari Subagyo, Rie Shimizu, Izumi Orita, Toshiaki Fukui. J Biosci Bioeng., 132(5), 479-486(2021)
Methylotrophic bacterium-based molecular sensor for the detection of low concentrations of methanol. Viviane Carnier Casaroli, Izumi Orita, Shiori Katayama, Hiroya Yurimoto, Yasuyoshi Sakai, Toshiaki Fukui. J. Biosci Bioeng., 132(3), 247-252(2021)
A study on the effects of increment and decrement repeated fed-batch feeding of glucose on the production of poly(3-hydroxybutyrate) [P(3HB)] by a newly engineered Cupriavidus necator NSDG-GG mutant in batch fill-and-draw fermentation. Nazila Biglari, Izumi Orita, Toshiaki Fukui, Kumar Sudesh. J. Biotechnol., 307, 77-86(2020)
Modification of acetoacetyl-CoA reduction step in Ralstonia eutropha for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from structurally unrelated compounds. Mengxiao Zhang, Shunsuke Kurita, Izumi Orita, Satoshi Nakamura, Toshiaki Fukui. Microb. Cell. Fact., 18, 147(2019)
Two NADH-dependent (S)-3-hydroxyacyl-CoA dehydrogenases from polyhydroxyalkanoate-producing Ralstonia eutropha. Mutsumi Segawa, Cheng Wen, Izumi Orita, Satoshi Nakamura, Toshiaki Fukui. J. Biosci. Bioeng., 127, 294-300 (2019)
Enhancement of bioplastic polyhydroxybutyrate P(3HB) production from glucose by newly engineered strain Cupriavidus necator NSDG-GG using response surface methodology. Nazila Biglari, Marjan Ganjali Dashti, Peyman Abdeshahian, Izumi Orita, Toshiaki Fukui, Kumar Sudesh. 3 Biotech, 8, 330 (2018)
Compositional regulation of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by replacement of granule-associated protein in Ralstonia eutropha. Yui Kawashima, Izumi Orita, Satoshi Nakamura, Toshiaki Fukui. Microb. Cell. Fact., 14, 187 (2015)
New Insight into the Role of the Calvin Cycle: Reutilization of CO2 Emitted through Sugar Degradation. Rie Shimizu, Yudai Dempo, Yasumune Nakayama, Satoshi Nakamura, Takashi,bamba, Eiichiro Fukusaki, Toshiaki Fukui. Sci. Rep., 10, 11617 (2015)
Improved artificial pathway for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) with high C6-monomer composition from fructose in Ralstonia eutropha. Chayatip Insomphun, Huan Xie, Jun Mifune, Yui Kawashima, Izumi Orita, Satoshi Nakamura, Toshiaki Fukui. Metab. Eng., 27, 38-45 (2015)
Enhancement of glycerol utilization ability of Ralstonia eutropha H16 for production of polyhydroxyalkanoates. Toshiaki Fukui, Masaharu Mukoyama, Izumi Orita, Satoshi Nakamura. Appl. Microbiol. Biotechnol., 98, 7559-7568 (2014)
Biosynthesis of polyhydroxyalkanoate copolymers from methanol by Methylobacterium extorquens AM1 and the engineered strains under cobalt-deficient conditions. Izumi Orita, Kouta Nishikawa, Satoshi Nakamura, Toshiaki Fukui. Appl. Microbiol. Biotechnol., 98, 3715-3725 (2014)
Modification of β-oxidation pathway in Ralstonia eutropha for production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from soybean oil. Chayatip Insomphun, Jun Mifune, Izumi Orita, Keiji Numata, Satoshi Nakamura, Toshiaki Fukui. J. Biosci. Bioeng., 117, 184-190 (2014)
